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In this chapter we describe a strategy to circumvent immunological tolerance that can be used to generate high avidity CTL against self-proteins, including human tumor-associated antigens. High-mobility group protein box 1 (HMGB1), also know as amphoterin, is a nonhistone nuclear protein with well-established functions in gene transcription and homeostasis regulation into the cell nucleus. We validated our approach by exemplarily implementing a parallel-coordinate data visualization into our software and plan to integrate further algorithms for visual analytics and machine learning to support knowledge discovery in medicine in diverse ways.

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